BioLector XT
GET MO2RE DATA. NOW. With or without O2
The BioLector XT high-throughput microbioreactor enables real-time evaluation of biomass, pH, dissolved oxygen in the liquid phase (DO), fluorescence and other key cultivation parameters for aerobes and anaerobes— to quickly provide deep insights into your bioprocess development.
Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with non-invasive, pre-calibrated optical sensors.
Disposable 48-well MTPs enable online measurement of key cultivation parameters while an optional microfluidic module supports simultaneous, well-individual pH control and feeding.
This patented microfluidic technology eliminates manual liquid handling—no tubing/pipetting required, as everything is part of the gamma-radiated ready-to-use plate.
The BioLector XT high-throughput microbioreactor enables real-time evaluation of biomass, pH, dissolved oxygen in the liquid phase (DO), fluorescence and other key cultivation parameters for aerobes and anaerobes— to quickly provide deep insights into your bioprocess development.
Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with non-invasive, pre-calibrated optical sensors.
Disposable 48-well MTPs enable online measurement of key cultivation parameters while an optional microfluidic module supports simultaneous, well-individual pH control and feeding.
This patented microfluidic technology eliminates manual liquid handling—no tubing/pipetting required, as everything is part of the gamma-radiated ready-to-use plate.
BioLector XT Microbioreactor Features
| INNOVATIVE NEW GASSING LID | OPTIONAL MICROFLUIDIC MODULE | “PLUG-AND-PLAY” PLATE DESIGN | INTELLIGENT BIOLECTION SOFTWARE |
| Enables fed-batch experiments under anaerobic conditions | Unleashes full potential of the BioLector XT | Online data out of 48/32 parallel cultivations | Intuitive user interface supports multi-user environments |
| Gassing with O22within a range of 1% - 100% and with CO2 within 0% - 12 % | Complements online monitoring function with well-specific pH regulation/feeding | Customizable feeding strategies (batch, fed-batch)* | Free programming of all control parameters |
| Reduces gas consumption to a few mL/minute | Enables use of 2 feed lines per cultivation well | Control of pH on-the-plate with pre-calibrated optical sensors* | Open system enables live data downloads |
| Optional humidification of gases reduces evaporation | Microvalves allot liquids at nanoliter-scale | Small working volume (800 – 2400 μL) | Fast processor ensures rapid download of experiment data |
| *Functionality requires optional microfluidic module |
Additional features include:
- Capability to fully customize cultivation protocols to cover a broader spectrum of applications
- Enables free combination of different feeding and pH control strategies over one cultivation run
- Updated BioLection software provides an intuitive user interface designed for multi-user environments
- New gassing lid reduces gas consumption and acts as an air-tight anaerobic chamber that can be used with the microfluidic module, eliminating the need for anaerobic tents.
System Performance
- 32/48 parallel microreactions, optionally 16 reservoir wells
- Working volume: 800 – 2400 μL
- Pre-calibrated optical sensors for online pH and DO measurement
- Well-individual pH control and feeding
- Fully flexible feeding options: constant, linear, exponential or signal-triggered, with any combinations possible
- Broad range of kLa values (30 – 600 h-1)
- Continuous gas exchange and oxygen supply
- Strictly anaerobic cultivations possible, optionally with active pH control and feeding
- Equal power input into each reactor
- Defined engineering parameters and scalability
- Controlled gas atmosphere (CO2, O2)
- Temperature control (8 °C below ambient temperature to 50 °C) with active water cooling
"Plug and Play" Microtiter Plate Design
- Real-time kinetics out of 48/32 parallel cultivations
- Fully customizable and freely combinable actively regulated feeding strategies (batch, fed-batch, bolus, continuous)*
- Control of pH on-the-plate with precalibrated optical sensors*
- Flexible process control of pH, shaking, temperature and gassing
- Strictly anaerobic cultivations with feeding and pH control*
- Cultivation of highly oxygen-demanding strains with up to 100 % O2
- DO and signal-triggered feeding*
- Low pH measurements and control* available in the range of 4-6
- High-throughput and easy automation
- Broad range for biomass detection (equivalent to up to 250 OD600, 50 g/L CDW, measured with E. coli)
- Online biomass measurement requires no dilution
- Small working volume (800 – 2400 μL)
- No edge effects
- Continuous shaking operation (no artifacts)
- Defined mass transfer conditions
- Reliable scale-up to bench-top fermenters
- A valuable tool for PAT and QbD
*Note: functionality requires the optional microfluidic module
MO2RE flexibility for more applications
- Feeding strategy development
- Feeding rate optimization
- Media screening and optimization
- Cultivation parameter optimization
- pH profiling • Strictly anaerobic and microaerophilic cultivations in batch and fed-batch mode
- High-oxygen (up to 100 %) and high-carbon dioxide (up to 12 %) cultivations
- Cell line and strain screening
- Synthetic and systems biology
- Statistical design of experiments (DoE)
- Growth characterization
- High-throughput protein expression
- Enzyme and cell activity tests
- Functional genomics
- Proteomic studies
- Inhibition and toxicity tests
- Quality control
Online Measurement / Online Control
MO2RE control & more data for deeper insights
Online Measurement
- Biomass concentration
- pH value
- Dissolved oxygen (DO)
- NAD(P)H and Riboflavins
- Fluorescent molecules (e.g., GFP, YFP, DsRed)
- Shaking speed
- Temperature
- O2 in head space atmosphere
- CO2 in head space atmosphere
Online Control
- pH value (well-specific)
- Feeding (well-specific)
- Shaking speed
- Temperature
- Gas flow
- O2 in head space atmosphere
- CO2 in head space atmosphere
Optional Microfluidic Module helps BioLector XT microbioreactor do even MO2RE
- Unleashes the full potential of the BioLector XT microbioreactor
- Complements online monitoring function with well-specific pH regulation and feeding
- Enables use of 2 reservoir wells per 4 cultivation wells—with either 2 pH-adjusting solutions, 2 feed solutions or 1 of each
- Liquids allotted in nanoliter-scale through microvalves
