Opentrons OT-2
Get started automating your lab today
Lab automation has never been easier.
The OT-2 is a bench-top liquid handler designed to be accessible and flexible enough to automate many common applications.
Small Footprint, Big Impact
Fits on half a standard lab bench
Performs like robots costing 10x more
Choose your hardware and pipettes
11 deck slots, plus trash, and space for two swappable, single and 8-Channel pipettes
Magnetic, Temp, Thermocycler, Heater-Shaker, and HEPA Filter modules available
Use your existing labware and reagents
Reagent and consumables agnostic
Compatible with any ANSI/SLAS-compliant labware
Choose the protocol method that’s right for you
A protocol is the code that runs the robot through your experiment. We’ve built different ways to create a protocol that are accessible for everyone – from the automation rookie to the experienced Python coder.
Space for two pipettes
Reliable liquid transfers from 1–1000 µL with our swappable single- and 8-channel pipettes
Dimensions
63 cm x 57 cm x 66 cm / 25 in x 22.5 in x 26 in (W,D,H)
Takes up half of your standard lab bench
48 kg or 105.8 lb
Single- and 8-channel pipetting
2 pipette mounts, for a configuration of 1 or 2 single- or 8-channel pipettes. Pipettes are easily interchangeable.
11 deck slots enable countless configurations
Deck slots are compatible with standard SBS dimensions.
Deck also includes a removable trash bin.
Integrated video camera (Included for future or custom functionality)
Integrated audio system (Included for future or custom functionality)
Robot Power Input: 36 VDC, 6.1 A
Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
For additional power requirement information,
Rigid steel and CNC aluminum design
Recommended 20-24 °C
Recommended 40-60% RH
| Pipette | Distribute, one tip | Transfer, same tip | Transfer, new tip |
|---|---|---|---|
| P20 8-Channel | 0:22 | 1:35 | 4:19 |
| P300 8-Channel | 0:26 | 1:38 | 4:24 |
| P20 Single-Channel | 3:12 | 13:10 | 36:01 |
| P300 Single-Channel | 4:01 | 13:39 | 41:31 |
| P1000 | 5:54 | 15:21 | 44:50 |
| Pipette | Volume | Random Error | Systematic Error | ||||
|---|---|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||||
| P20 | 1 10 20 |
±15% ±2% ±1.5% |
0.15µl 0.2µl 0.3µl |
±5% ±1% ±0.8% |
0.05µl 0.1µl 0.16µl |
||
| P300 | 20 150 300 |
±4% ±1% ±0.6% |
0.8µl 1.5µl 1.8µl |
±2.5% ±0.4% ±0.3% |
0.05µl 0.6µl 0.9µl |
||
| P1000 | 100 500 1000 |
±2% ±1% ±0.7% |
2.0µl 5.0µl 7.0µl |
±1% ±0.2% ±0.15% |
1µl 1µl 1.5µl |
| Pipette | Volume | Random Error | Systematic Error | ||||
|---|---|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||||
| P20 | 1 10 20 |
±20% ±3% ±2.2% |
0.2µl 0.3µl 0.44µl |
±10% ±2% ±1.5% |
0.1µl 0.2µl 0.3µl |
||
| P300 | 20 150 300 |
±10% ±2.5% ±1.5% |
2.0µl 3.75µl 4.5µl |
±4% ±0.8% ±0.5% |
0.8µl 1.2µl 1.5µl |
Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Interfaces with any product that has an API
Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
Use Opentrons Tips and Opentrons Filter Tips
Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
Use common labware already in our Labware Library, or add your own. If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
One year warranty against manufacturer defects
Stress tested to last a minimum of 3 years of full-time use
Certifications complete (CE, FCC, NRTL, CB, ISO 9001)
Not certified/validated (IVD, GMP)
Tip Handling
- Pick up tip
- Drop tip
- Return tip
- New tip (always, never, once)
- More…
Liquid Control
- Aspirate
- Dispense
- Aspirate speed
- Dispense speed
- Blowout
- Touch tip
- Mix
- Air gap
Utility
- Move to
- Delay
- User-specified pause
- Homing
- Comment
Transfer
- Basic transfer
- Volumes > Pipette Volume
- Distribute
- Consolidate
Variable Settings
- Speed
- Volume
- Count
Other
- Dispose
General Liquid Handling
The quickest way to save time in the lab. You can easily automate a range of basic liquid handling tasks on the OT-2 pipetting robot, including tube and microplate filling, serial dilution, cherry picking, normalization and sample pooling. Choose from a range of verified protocols in our Protocol Library, create your own, or ask our Applications Engineering team to create one for you.
PCR Setup
Another popular application for automation, especially if you’re prepping a lot of samples. You can use the OT-2 pipetting robot to combine samples and PCR mastermix in the reaction plate that will go into the thermocycler or qPCR machine.
Protein Purification
The most cost-effective way to automate small-scale protein purification, proteomics sample processing, and enzymatic and chemical lysis. We currently offer two approaches to automate on the OT-2 pipetting robot – dual flow chromatography-based automation, and magnetic bead-based automation.
