NanoDrop Eight UV-Vis Spectrophotometer
Thermo Scientific NanoDrop Eight UV-Vis Spectrophotometers are used in academic, biopharmaceutical, core and bioproduction facilities, to measure between 1 and 8 microvolume nucleic acid and protein samples simultaneously. Increase the chances of success in your analyses with efficient analysis of your sample quantity and quality using the Thermo Scientific™ NanoDrop™ Eight UV-Vis spectrophotometer. Ideal for larger number of nucleic acid and protein samples. NanoDrop Eight measures 8 samples at a time in less than 20 seconds. Software identifies sample contaminants and reports true sample concentration with the built-in Thermo Scientific™ Acclaro™ Sample Intelligence technology. Optional software allows you to more easily comply with 21 CFR Part 11 regulation.
NanoDrop Eight Spectrophotometer features:
- Wide spectral range (190- 850nm) for measuring a variety of samples types:
- DNA and RNA (260nm)
- Purified protein (280nm)
- Toxicology assays and industrial dyes (490nm)
- Gold nanoparticles (520nm)
- Colorimetric protein assay (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
- Optical Density measurements (600 nm)
- No dilutions required
- Only requires 1-2 µL of your sample
- Calculates sample purity ratios (260/280 nm and 260/230 nm)
- Detects contaminants in sample and recommends corrected quantitiy of measured nucleic acid or protein (Acclaro™ Sample Intelligence technology)
- Measure up to 96 samples in less than six minutes
- Choose to measure form 1 to 8 samples in one measurement cycle
- Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
- Patented sample retention system automatically optimizes pathlength to accommodate low and high concentrations (2 to 10000 ng/µL dsDNA)
- User-friendly software that includes custom methods and data export capabilities
Position Illuminator
When measuring samples in a 96-well format on the Thermo ScientificNanoDrop 8000 Spectrophotometer, an eight-channel pipettor is used to draw 1 ul samples from a microtiter plate which are then dispensed onto the eight optical measurement surfaces.
The Sample Position Illuminator keeps track of the samples to be measured by lighting up wells from beneath the plate. This helps the user readily identify which wells to draw from for the next measurement, thus reducing fatigue and human error.
The sample position illuminator is simple to use. Prior to measurement, the user selects the samples to be measured from the on screen position indicator (selected wells are in green).
The sample position illuminator then lights up the corresponding wells of the plate that are to be measured:
The user then simply draws from the illuminated wells.
Additional Features:
- Auto advance: This increases measurement throughput by automatically
- lighting up the next column to be measured.
- "Cherry picking": User can select any single or multiple wells which are
- then lit up by the sample position illuminator.
- Measurement limits: The user can define upper and lower concentration
- (or absorbance) limits such that a well will begin to flash if the
- measurement falls out of a defined range (e.g. 100ng/ul ±20ng/ul).
Performance
Data from studies using the Thermo Scientific NanoDrop Eight Spectrophotometer are below:
| Sample size | 1 µl |
| Sample Number | up to 8 |
| Path lengths | 1, 0.2 és 0.1 mm |
| Light Source | Xenon flash lamp |
| Detector Type | 2048-element CMOS linear image sensor |
| Wavelength range | 190-850 nm |
| Wavelength accuracy | 1 nm |
| Wavelength resolution | 1.8 nm (FWHM at Hg 254 nm) |
| Recommended Sample Volume, Nucleic acid | 1 µl |
| Recommended Sample Volume, Proteins | 2 µl |
| Absorbance Accuracy | 3% (at 0.97 absorbance at 302 nm) |
| Absorbance Range | 0.03-200 (10 mm equivalent absorbance) |
| Detection Limit | 2 ng/µl (dsDNA) |
| Maximum Concentration | 10000 ng/µl (dsDNA) |
| Measurement Cycle Time | 20 seconds (for 8 samples) |
| Dimensions (footprint) | 24 X 33 X 17 cm |
| Weight | 3.7 kg |
| Operating Power Consumption | 15 W |
| Standby Power Consumption | 3 W |
- The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
- Measuring Viability Decline in Animal Cell Suspension Cultures Using the Thermo Scientific NanoDrop Spectrophotometers.
- Nanoparticle Characterization Resources for the NanoDrop Spectrophotometer
- Quantification of Gold Nanoparticles
- Differences in Bacterial Optical Density Measurements between Spectrophotometers
- Activity Measurements of Pentose Phosphate Pathway Enzymes Using Kinetics
- Quality Control during Library Construction for de novo Next-Gen Sequencing Platforms
- Generation of a Standard Curve Based Custom Method using the NanoDrop instruments: Lowry Assay Case Study
- Quality Control of Industrial Dye Products Using Absorbance Spectroscopy
- Detection and Avoidance of Polysaccharides in Plant Nucleic Acid Extractions
- Quality Control of Small Molecule Pharmaceuticals using Spectrophotometry
- Pierce 660 nm Micro-Volume Assay Advantages
- Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies
- Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence (Poster)
- Microvolume Quantification of Nucleic Acids in Molecular Diagnostics (Poster)
- Small Molecule Crystallography
- Microgenomic Expression Profiling
- Quantitative Real Time RT PCR
- Nucleic Acid and Fluorescent Dye Concentration Measurement
- HLA Typing: Template Quality Control for SSP and SSO
