SpectraMax M Series Multi-Mode Microplate Readers
Every SpectraMax® M Series Multi-Mode Microplate Reader does UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes, standard. After that, you're free to choose the reader that gives you the most empowering combination of features for your lab. Choose one that adds glow luminescence, time-resolved fluorescence, fluorescence polarization, or, in the case of our top-of-the-line M5e, all of these, plus HTRF.
If you need one of these readers now, but you're not certain about your future requirements, choose one of our upgradeable M3, M4 or M5 Readers.
All M Series Readers accept cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Standard features include UV-visible absorbance in tunable 1.0 nm increments up to 4.0nm, and fluorescence intensity with spectral scanning across the wavelength range in 1 nm increments and up to four wavelength pairs per protocol supporting FRET based assays.
Patented AutoPMT Optimization System
- The patented AutoPMT optimization system of the SpectraMax M5 Microplate Reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays so that low, medium, and high signals can be captured from the same plate. This calibration against an internal standard provides an additional benefit in being able to confidently compare relative fluorescence units (RFUs) of individual samples across plates and readers.
Patented PathCheck Pathlength Measurement Technology
- Offers the capability to measure the depth (optical pathlength) of samples in a temperature independent manner using the patented PathCheck® Technology. With SoftMax Pro® Software, the PathCheck Technology automatically normalizes the well absorbance.
Comprehensive Reader Validation
- Molecular Devices microplate readers have the most complete level of product validation and compliance of any system on the market. A variety of tools are available to users to meet users' diverse validation needs. Molecular Devices provides the complete solution covering the instrument and software.
SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
- IQ/OQ/PQ documentation
- SoftMax Pro Data Acquisition & Analysis Software validation package
- Software tools for FDA 21 CFR Part 11 compliance
Automating with Ease
- Offers seamless integration with the StakMax® Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for SpectraMax Microplate Readers and AquaMax® Microplate Washers. SpectraMax M5 Microplate Reader has an automation-compatible reader design for integration into automated platforms. Molecular Devices microplate readers have been integrated into all major automation platforms.
Assay Kits
-
We offer optimized assay kits for use with the SpectraMax M Series readers.
SoftMax® Pro Data Acquisition & Analysis Software, Molecular Devices industry leading all-in-one data acquisition and analysis software, offers:
- The most extensive library of "Ready-to-Run" application protocols to get you started faster
- Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
- Advanced calculations including non-linear fitting metrics for in-depth analysis
- The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
- Proven integrations with leading automation and LIMS platforms for partnered solutions
- Pre-written SpectraDrop™ Plate protocols enabling micro volume applications measurements
- Seamless integrated with the StakMax® Microplate Handling Stacker
SpectraMax M2 / m2E
|
General Specifiations |
|||||||||||||||||||||||||||||
|
Dimensions (in.) |
8.6 (H) x 22.8 (W) x 15 (D) |
||||||||||||||||||||||||||||
|
Dimensions (cm) |
22 (H) x 58 (W) x 38 (D) |
||||||||||||||||||||||||||||
|
Weight |
35 lbs. (15.9 kg) |
||||||||||||||||||||||||||||
|
Power consumption |
< 125 watts |
||||||||||||||||||||||||||||
|
Power source |
100-240 Vac, 3 A, 50/60 Hz |
||||||||||||||||||||||||||||
|
Absorbance Photometric Performance |
|||||||||||||||||||||||||||||
|
Wavelength range |
200-1000 nm |
||||||||||||||||||||||||||||
|
Wavelength selection |
Monochromator, tunable in 1.0 nm increments |
||||||||||||||||||||||||||||
|
Wavelength bandwidth |
≤ 4.0 nm |
||||||||||||||||||||||||||||
|
Wavelength accuracy |
±2.0 nm |
||||||||||||||||||||||||||||
|
Wavelength repeatability |
±0.2 nm |
||||||||||||||||||||||||||||
|
Photometric range |
0-4.0 OD |
||||||||||||||||||||||||||||
|
Photometric resolution |
0.001 OD |
||||||||||||||||||||||||||||
|
Photometric accuracy (microplate) |
< ±0.006 OD ±1.0%, 0-2 OD |
||||||||||||||||||||||||||||
|
Photometric accuracy (cuvette) |
< ±0.005 OD ±1.0%, 0-2 OD |
||||||||||||||||||||||||||||
|
Photometric precision |
< ±0.003 OD ±1.0%, 0-2 OD |
||||||||||||||||||||||||||||
|
Baseline flatness |
< 0.001 OD |
||||||||||||||||||||||||||||
|
Stray light |
< 0.05% @ 230 nm |
||||||||||||||||||||||||||||
|
Fluorescence Photometric Performance (M2) |
|||||||||||||||||||||||||||||
|
Dual monochromators |
1 nm increments |
||||||||||||||||||||||||||||
|
EX |
250-850 nm |
||||||||||||||||||||||||||||
|
EM |
250-850 nm |
||||||||||||||||||||||||||||
|
Bandwidth (EX, EM) |
9, 9 nm |
||||||||||||||||||||||||||||
|
Optimized sensitivity (top-read) |
5 pM (fluorescein) |
||||||||||||||||||||||||||||
|
Guaranteed sensitivity (top-read)** |
15 pM (fluorescein) |
||||||||||||||||||||||||||||
|
Fluorescence Ph Molecular Devices Assay Partnerships Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. To support these assays, we provide application notes as well as ready-to-run protocols in our SoftMax Pro Software.
SpectraMax® M Series Multi-Mode Microplate Readers
Related Video
Related Collateral
Applications
Poster
Number of Citations*: 11,100
Quantification of Catecholamine Uptake in Adult Cardiac MyocytesEF Dahl, CD Wright, TD O'Connell - Nuclear G-Protein Coupled Receptors, 2015 - Springer ... 1. FlexStation 3 Benchtop Multi-Mode Microplate Reader. 2. SpectraMax M5 Multi-Mode Microplate
[HTML] Determining Antioxidant Activities of Lactobacilli Cell-Free Supernatants by Cellular Antioxidant Assay: A Comparison with Traditional MethodsJ Xing, G Wang, Q Zhang, X Liu, Z Gu, H Zhang… - PloS one, 2015 - dx.plos.org ... The control sample contained deionized water instead of the sample solution. The
| |||||||||||||||||||||||||||||

The SpectraMax microplate readers support a wide variety of assay categories including


























