SpectraMax i3x Multi-Mode Detection Platform
The SpectraMax® i3x Platform from Molecular Devices is a multi-mode microplate reader system that evolves with your future needs and offers an unlimited breadth of application possibilities.
Future ready
The SpectraMax i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system.
Protect your initial investment — purchase a system with the flexibility to add novel detection capabilities without the need for service engineers or costly system downtime. The SpectraMax i3x reader grows with you as your research areas expand.
Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform.
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Expanded dynamic range |
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User-upgradeable applications |
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One complete solution |
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Investigate every aspect of a cellular pathway
From imaging of cell confluence and viability under different treatment conditions to quantitation of nucleic acids and protein to western blot analysis, a wealth of new knowledge is captured using a single instrument. One versatile software package powers data acquisition and analysis, from raw data to publishable results. See more and do more cellular to protein analysis using a single detection system. |
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Live cell imaging The SpectraMax MiniMax 300 Imaging Cytometer option allows for live cell images and analysis (top, left). The ScanLater Western Blot Detection Cartridge enables protein detection (top, right). |
Visualize cells with
Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The plate is set up for reading and images are acquired according to specified parameters. Cells in each image are identified by SoftMax® Pro Software and cell-by-cell statistics are collected. Data are then analyzed and visualized in different graphical representations. |
Technical specification of the SpectraMax i3x are identical to those of the SpectraMax i3
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General Specifications |
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| Dimensions (W x H x D) |
12.63 (H) x 15.38 (W) x 23.38 (D) (in) 42.23 (H) x 39.05 (W) x 59.37 (D) (cm) |
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| Weight | 68.3 lbs (31 kg) | |
| Power consumption | < 200 watts | |
| Power source | 100-240 VAC, 2 A, 50/60 Hz | |
| Robotic-compatible | Yes | |
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General Photometric Performance |
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| Plate formats | 6 to 1536 wells* | |
| Light source | Spectral Fusion Illumination (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges) | |
| Reading capabilities | Microplates, cuvettes (via adapter), low volume samples (via SpectraDrop, Micro-Volume Microplates) | |
| Detectors | PMT and/or photodiode | |
| Shaking | Linear and orbital | |
| Dual Auto Injectors | Available October 1, 2015 | |
| Temperature control | 4°C above ambient to 45°C | |
| Temperature uniformity | ± 0.75°C | |
| Temperature accuracy | ± 1°C at 37°C set point | |
| Environmental control | Gas quick connect | |
| Endpoint reading | all modes | |
| Kinetic reading | all modes | |
| Spectral scanning | Absorbance, Fluorescence, Luminescence | |
| Well scanning | Over 20 by 20 in all modes | |
| Wavelength selection | 1.0 nm increments | |
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Standard Read Times (minutes:seconds)** |
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| 96 wells | 384 wells | |
| Absorbance | 0:30 | 1:40 |
| Fluorescence Intensity | 0:25 | 1:25 |
| Luminescence | 0:30 | 1:15 |
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Absorbance Photometric Performance |
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| Wavelength range | 230-1000 nm | |
| Wavelength bandwidth | 4.0 nm | |
| Wavelength accuracy | ± 2.0 nm | |
| Wavelength repeatability | ± 1.0 nm | |
| Photometric range | 0-4.0 OD | |
| Photometric resolution | 0.001 OD | |
| Photometric accuracy | < ± 0.010 OD ± 1.0%, 0-2OD | |
| Photometric precision | < ± 0.003 OD ± 1.0%, 0-2 OD | |
| Stray light | < 0.05% @ 230 nm | |
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Fluorescence Intensity Performance |
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| Wavelength range | 250-850 nm | |
| Wavelength selection | 1.0 nm increments | |
| Bandwidth | Adjustable: 9/15 nm (ex), 15/25 nm (em) | |
| Dynamic range | > 6 logs | |
| Top sensitivity (fluorescein) | ||
| Optimized | ||
| 96 wells | 0.5 pM | |
| 384 wells | 1 pM | |
| Bottom Sensitivity (Fluorescein) | ||
| Optimized | ||
| 96 wells | 5 pM | |
| 384 wells | 5 pM | |
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Luminescence Performance |
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| Wavelength range | 300-850 nm | |
| Wavelength selection | Choice of simultaneous detection of all wavelengths or selection in 1.0 nm increments | |
| Dynamic range | > 6 decades | |
| Cross-talk | < 0.4% in white 96- and 384-well microplates | |
| Sensitivity (ATP-Glow) | ||
| Optimized | ||
| 96 wells | 3 pM | |
| 384 wells | 6 pM | |
* 1536 detection available via detection cartridges.
** With 6 flashes in absorbance and 3 flashes in fluorescence mode, and 0.1 sec/well integration in 96-well luminescence mode and 0.04 sec/well integration in 384-well luminescence mode.
With the SpectraMax i3x Multi-Mode Detection Platform, researchers are able to use a wide range of detection cartridges, like the ScanLater Western Blot System, that provide increased plate reader functionality. The SpectraMax MiniMax 300 Imaging Cytometer option is available for cellular imaging assays and the SpectraDrop™ Micro Volume Microplates for low volume DNA, RNA and protein sample prep. Now, all of your needs are covered with a single plate reader.
Representative Applications
- AlphaScreen®
- Apoptosis
- BRET
- Cellular imaging assays
- Cell cycle analysis
- Cell proliferation, viability, cytotoxicity
- Enzyme activity
- Epigenetics
- Fast kinetic calcium mobilization assays
- Fluorescent protein expression
- FRET
- Genotyping
- GPCRs
- GTP binding
- Hormones
- HTRF®
- Immunoassays
- Ion channels
- Membrane potential assays
- Micro Volume (Low volume) Applications
- Molecular interactions
- Nuclear receptor
- Nucleic acid and Protein quantitation
- Reporter assay
- Time-Resolved Fluorescence (TRF)
- Western blot detection
SpectraMax® i3x Multi-Mode Microplate Reader System
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System Options |
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| Product | Part Number |
| SpectraMax i3x Multi-Mode Microplate Reader with SoftMax Pro Software | i3x |
| SpectraMax MiniMax 300 Imaging Cytometer | 5024062 |
| SoftMax Pro 6.x GxP Software | by license bundle |
| SpectraTest (ABS1) Absorbance Validation Plate | 0200-6117 |
| SpectraTest (LM1) Luminescence Validation Plate | 0200-6186 |
| SpectraTest (FL1) Fluorescence Validation Plate | 0200-5060 |
| SpectraDrop Micro-Volume Starter Kit | 0200-6262 |
| SpectraDrop Micro-Volume HTS Kit | 0200-6267 |
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SpectraMax Detection Cartridge Options |
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| Product Name | Part Number |
| AlphaScreen Detection Cartridge (384 STD) | 0200-7017POS |
| AlphaScreen Detection Cartridge (384 HTS) | 0200-7018POS |
| AlphaScreen Detection Cartridge (1536 HTS) | 0200-7019POS |
| HTRF Detection Cartridge | 0200-7011POS |
| TRF Detection Cartridges | 0200-7008POS |
| Fluorescence Polarization Detection Cartridge (Fluorescein) |
0200-7009POS |
| Fluorescence Polarization Detection Cartridge (Rhodamine) | 0200-7010POS |
| Luminescence Detection Cartridge (Glow for 96-well plates) |
0200-7014POS |
| Luminescence Detection Cartridge (Glow 96- and 384-well plates) |
0200-7015POS |
| Luminescence Detection Cartridge (Glow 96-, 384-, and 1536-well plates) |
0200-7012POS |
| Dual Glow Luminescence Detection Cartridge (BRET2) |
0200-7016POS |
| Fluorescence Intensity Detection Cartridge (Chroma-Glo) |
0200-7013POS |
| Fluorescence Intensity Detection Cartridge (Courmarin-Fluorescein) |
0200-7002POS |
| Fluorescence Intensity Detection Cartridge (Fluorescein-Rhodamine) |
0200-7003POS |
| Fluorescence Intensity Detection Cartridge (Cy3-Cy5) |
0200-7004POS |
| Fluorescence Intensity Detection Cartridge (CFP-YFP) |
0200-7005POS |
| Fluorescence Intensity Detection Cartridge (GeneBLAzer) |
0200-7006POS |
| ScanLater Western Blot Detection Cartridge | 0200-7027 |
Scan Later Western Blot Detection System 
The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3x and Paradigm® Multi-Mode Platforms. The ScanLater Western blot system simplifies the traditional western blot protocol by employing a europium-labelled secondary antibody which is then read by Time-Resolved Fluorescence (TRF). This substrate free detection method enables membrane scanning up to 30 days after the final wash step. The assay demonstrates sensitivity in the high femtogram, comparable to traditional chemiluminescence western blot methods. Leveraging this novel technology, researchers can:
- Add western blot detection to their microplate reader within two minutes
- Eliminate time-dependent substrate addition steps
- Use one software platform, SoftMax® Pro Software to integrate both microplate and western blot detection assays
Features
- User-installable cartridge for the SpectraMax i3x and Paradigm Multi-Mode Platforms
- TRF detection using Europium-labelled secondary antibody reduces background while increasing dynamic range.
- Digital photon counting provides TRF counts unaltered for a high level of data integrity
- Europium resists photo bleaching and is stable for weeks, enabling re-use of blots
- Compatible with goat anti-rabbit, anti-mouse or streptavidin labeled antibodies
- SoftMax Pro acquisition and analysis software that provides built-in protocols for Western Blot reads and easy-to-use image analysis and accurate quantification tools
SpectraMax MiniMax 300 Imaging Cytometer
The SpectraMax MiniMax 300 Imaging Cytometer option, which is field-upgradeable for any SpectraMax i3x base system, provides researchers with cellular analysis capability without the need to invest in complex imaging systems.
Benefits
- Obtain cell-based data in addition to well-based intensity readings in the same instrument
- Eliminate cell staining for cell counting and confluency with StainFree Technology
- Perform multi-parametric assays and get rich, cell-by-cell statistics
- Easily measure low cell concentration samples
Features
- Brightfield and fluorescent green and red detection channels
- Cytotoxicity, protein expression and cell proliferation assays in addition to ratio-metric assays like live-dead and transfection efficiency
- ANSI/SBS conformant plate compatibility
- Quick cellular identification and segmentation with SoftMax® Pro Microplate Data Acquisition & Analysis Software
StainFree Cell Detection Technology
The patent-pending StainFree Cell Detection technology on the SpectraMax MiniMax 300 Imaging Cytometer allows users to perform cell proliferation, cytotoxicity, and other assays without nuclear stains like DAPI or live cell dyes that are toxic to cells in the long term. Using StainFree Cell Detection Technology, users can:
- Count cells and estimate confluence without fluorescent dyes
- Monitor cell growth without disrupting cells
- Set-up imaging assays quickly and easily with SoftMax Pro Software
Related Videos
Brochure
Applications
- Luminescent cell viability and cytotoxicity assays on
the SpectraMax i3x Multi-Mode Microplate Reader
Related eBooks
- Advance Detection of Cytotoxicity: A wide range of solutions for predicting cytotoxicity earlier in your drug discovery process
The resources listed below were created using the SpectraMax i3.
Technical specifications for the SpectraMax i3x are similar to those of the SpectraMax i3. For luminescence, the LLD specification for the SpectraMax i3x is 5x lower (better) than for the SpectraMax i3.
Applications
- Compound effects upon calcium transients in beating Axiogenesis Cor.4U human iPS cell-derived cardiomyocytes
- Complete cAMP workflow solution using the CatchPoint cAMP Fluorescent Assay Kit
- Complete cGMP workflow solution using the CatchPoint cGMP Fluorescent Assay Kit
- Assessing mitochondrial toxicity on the SpectraMax i3 Multi-Mode Detection Platform with SpectraMax MiniMax 300 Imaging Cytometer
- HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
- Optimizing GFP transfection with the SpectraMax i3 Multi-Mode Detection Platform
- IMAP Phosphodiesterase Assays on SpectraMax Multi-Mode Microplate Readers
- StainFree Technology for Cellular Image Analysis without Fluorescent Dyes
- Detection and Quantitation of Protein with ScanLater Western Blot Detection System
- Monitoring Cytotoxicity on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
- Cardiomyocyte Beating and Cytotoxicity Assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
Posters
- HTRF Technology on the SpectraMax i3 Multi-Mode Platform
- Complex Cell-based Assays with a Novel Imaging Cytometry System and Object Recognition Software
Related Webinars
- StainFree Cell Detection Technology
- Novel In Vitro Assay Tools for Cardiac Toxicity and Discovery
Number of Citations*: 50
A quantum dot nano-carrier system for targeted drug delivery
(Veeco, NanoScope V). A calibration curve of fluorescence intensity versus particle concentration
was performed using a 96 well plate reader (Molecular Devices, SpectraMax i3). ...
Multiple Phosphatases Regulate Carbon Source-Dependent Germination and Primary Metabolism in Aspergillus nidulans
using a SpectraMax I3 spectrometer (Molecular Devices). Trehalose measurements
in germinating conidia. Trehalose was extracted from 5×10 ...
[HTML] Enhanced efficacy with azacytidine and oncolytic BHV-1 in a tolerized cotton rat model of breast adenocarcinoma
Reader (Molecular Devices, Sunnyvale, CA) and the fold change in fluorescence
relative to untreated, uninfected controls was calculated. Data ...
[PDF] Benchmarks
300 Imaging Cytometer (Molecular Devices, Sunnyvale, CA) using the included SoftMax Pro
software (v6.3). The software is set to utilize the imaging read mode and end-point read ...
Transcriptome Assessment Reveals a Dominant Role for TLR4 in the Activation of Human Monocytes by the Alarmin MRP8
coelentrazine (Promega) and subsequent measurement of luminescence with a
SpectraMax i3 plate reader (Molecular Devices). ER-Hoxb8 ...
Binding Characteristics and Protective Capacity of Cyanidin‐3‐Glucoside and its Aglycon to Calf Thymus DNA
Corp.). Fluorescence spectra analysis. The fluorescence spectra of the samples were measured
by a SpectraMax® I3 (Molecular Devices LLC, Wals, Austria). ...
Circulating Muramyl Dipeptide Is Negatively Associated with Interleukin-10 in the Frail Elderly
Cell-free DNA (cfDNA) was measured using the QuantiFluor dsDNA system (Promega, WI, USA)
and a SpectraMax i3 plate reader (Molecular Devices, CA, USA). ...
Equine platelet lysate as an alternative to fetal bovine serum in equine mesenchymal stromal cell culture–too much of a good thing?
consisting of PBS7 with 10% resazurin8. Plates were incubated at 38.5°C for 4 h and read
by plate reader (SpectraMax i3 Multi-Mode microplate reader)9. ...
Synergistic effect of HMGB1 knockdown and cordycepin in the K562 human chronic myeloid leukemia cell line
was added into each well and the cells were cultured for 1 h in 5% CO2 at 37˚C. Finally, optical
density values (OD) were read at 450 nm using a SpectraMax® i3x microplate reader ...
[HTML] A Yeast/Drosophila Screen to Identify New Compounds Overcoming Frataxin Deficiency
at 30°C for 3 days, and the cell density was measured by reading the optical density ( )
using a SpectraMax i3 microtiter plate reader (Molecular Device). ...
* as of June 25, 2015. Source: Google Scholar. Search results include "SpectraMax i3" and "SpectraMax i3x".
