NanoDropUltra UV-Vis & Fluorescence Spectrophotometer
A glove-compatible touchscreen offers easy access to preloaded software applications and enables purity and concentration assessments in seconds. Built-in Acclaro Sample Intelligence Technology helps identify and correct for the contaminants present. Make informed decisions on sample quality and improve your success in downstream applications with NanoDrop technology.
NanoDrop Ultra instruments introduce a suite of innovative features designed to enhance your laboratory workflow. Key highlights include an integrated qPCR calculator that precisely determines the sample volume required for downstream applications, and the optional Acclaro Pro software, which incorporates an advanced algorithm for highly accurate quantification of concentrated samples. Additionally, a diagnostic scheduler alerts you when a diagnostic test is due, ensuring consistent performance. These, along with numerous other cutting-edge features, make NanoDrop Ultra instruments an indispensable tool for modern laboratories.
Acclaro Sample Intelligence Technology
Acclaro technology allows you to:
Verify sample quality in seconds — identify sample contaminants and obtain corrected concentration results
Access information through alerts — receive instant feedback about sample quality with on-demand technical support and guided troubleshooting
Rely on accurate results — ensure measurement integrity with embedded sensor and digital image analysis
Pioneering pedestal technology
Simply pipet a drop of your DNA, RNA, or protein sample on the pedestal and pull down the arm; results appear in seconds
Advanced sample intelligence
By analyzing absorbance across the full spectrum, Acclaro Sample Intelligence software can identify contaminants and correct quantitation results before you use them in downstream applications
Rapid time to results
Results appear within seconds of initiating a measurement, and Auto-Blank and Auto-Measure settings can further streamline your workflow
Streamlined multi-sample processing
With instant Auto-Measure and Auto-Blank capabilities
Tiny sample sizes
Requires only 1–2 µL of sample without dilution, even for highly concentrated samples
Differentiates DNA from RNA
Acclaro software uses full-spectrum data to tease apart mammalian DNA from RNA and calculate corrected nucleic acid concentrations
Your dependable lab partner
Since their introduction in 2001, NanoDrop spectrophotometers have become indispensable in many labs, accumulating over 55,000 scientific citations
In addition, the NanoDrop Ultra spectrophotometers and fluorometers offer indispensable features that make it a reliable, easy-to-use laboratory workhorse for many sample and assay types, including regulated environments. To learn more, see the sections below.
Expanded dynamic range
Measure from 1 ng/µL up to 27,500 ng/µL dsDNA or 0.02 mg/mL to 400 mg/mL IgG without dilution.
Optional Acclaro Pro software for accurate high concentration measurements
With the use of the optional Acclaro Pro software obtain absorbance measurements within 5% error up to 550 Abs (400 mg/mL IgG or 22,000 ng/µL ssDNA).
Diagnostic Scheduler
Use the built-in Diagnostic Schedule application to alert you when a diagnostic test is overdue, ensuring consistent performance of the instrument.
Cuvette option
Choose the NanoDrop Ultra or Ultra FL instrument for sample pedestal only or the NanoDrop UltraC or UltraC FL instrument for both pedestal and cuvette to expand the limit of detection to 0.2 ng/µL dsDNA or 0.006 mg/mL IgG.
Advanced cloud connectivity
Export data directly to popular Cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
Optional on-board SciVault 2 software for compliance
On-board Thermo Scientific SciVault 2 Software aids compliance with FDA 21 CFR Part 11 electronic recordkeeping regulations with subsequent seamless integration into your LIMS environment using RESTful API directly on the local instrument. SciVault 2 software can also be installed on a connected computer if desired.
All-in-one absorbance and fluorescence analysis
The NanoDrop Ultra FL and NanoDrop UltraC FL instruments are capable of both UV-Vis spectroscopy and fluorometry. The capable NanoDrop Ultra dsDNA (BR/HS) and RNA (HS) Fluorescence Assays allow for a more sensitive quantification of dsDNA and RNA and can detect down to 0.2 ng/µL dsDNA.
Wide spectral range
Measure the full UV-Vis spectrum from 190–850 nm, allowing a range of assays as shown in the table below
Routine and custom embedded applications
DNA, RNA, and protein quantification, full spectral data, purity ratios (A260/A280, A260/A230), and more
Local or PC control software
Operate your instrument from the glove-compatible touchscreen or your USB-connected PC, including custom methods and data export capabilities
Integrated qPCR Recipe Calculator
Fully integrated qPCR recipe calculator to save time, optimize efficiency, and achieve reliable downstream results with precise reaction setup.
Elevated Sample Insight
Software with the ability to differentiate between DNA and RNA for samples of mammalian, plant, and bacteria origin.
| Category | Attribute | Description |
| Pedestal Spectrophotometric | Minimum Sample Size | 1 μL |
| Sample Number | 1 | |
| Limit of Detection | 1 ng/μL (dsDNA), 0.06 mg/mL (BSA) | |
| Maximum Concentration | 27,500 ng/μL (dsDNA), 820 mg/mL (BSA) | |
| Pathlength(s) | 0.030 to 1.0 mm automatically adjusting | |
| Measurement Repeatability1 | 0.002 A (1.0 mm path) or 1% CV, whichever is greater | |
| Photometric Range | 0.02 - 550 Abs (10 mm equivalent) | |
| Photometric Accuracy2 | 3% at 0.97 A, 302 nm, 23 ± 2C° | |
| Measurement & Data Processing Time | ≤ 7 sec | |
| Acclaro Pro - Photometric Accuracy | 5% up to 550 Abs | |
| Acclaro Pro - Measurement & Data Processing Time | ≤ 30 sec | |
| Cuvette Spectrophotometric | Limit of Detection | 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA) |
| Maximum Concentration | 75 ng/μL (dsDNA), 2 mg/mL (BSA) | |
| Photometric Range | 0.004 - 1.5 Abs | |
| Pathlength(s) | 10 mm, 5 mm, 2 mm, 1 mm | |
| Beam Height (Z-Height) | 8.5 mm | |
| Temperature Control | 37.0 °C ± 0.5 °C | |
| Strirring | 9 speeds | |
| Absorbance-Based Optics | Light Source | Xenon flash lamp |
| Detector Type | 2048-element CMOS linear image sensor | |
| Wavelength Range | 190–850 nm | |
| Wavelength Accuracy | ±1 nm | |
| Spectral Resolution | ≤1.8 nm (FWHM at Hg 254 nm) | |
| Pedestal Fluorescence | Minimum Sample Size | 2 μL |
| Limit of Detection | Assay Specific3 | |
| Maximum Concentration | ||
| Measurement & Data Processing Time | ≤ 20 sec for Blue LED; ≤ 40 sec for Red LED | |
| Fluoresence-Based Optics | LEDs | Blue (max ~470 nm) and Red (max ~635 nm) |
| Excitation Filters | Blue (430-495 nm) and Red (600-645 nm) | |
| Characteristics | Footprint (W × D × H) | 32 × 18 × 28 cm (12.6 × 7.1 × 11.0 in.) |
| Weight | 4.1 kg | |
| Sample Pedestal | 303 stainless steel and quartz fiber | |
| Cuvette Position | Optional (with stirring) (NanoDrop UltraC and NanoDrop UltraC FL only) | |
| Operating Voltage | 12 V (DC) | |
| Power Consumption | 7W at Idle; 15W at Working Conditions | |
| Instrument Control | Built-in touchscreen or PC control with free PC software | |
| 21 CFR Part 11 Compliance | Yes, optional software for use on either PC or local instrument | |
| System | Applications Support | Nucleic Acid A260, A260/A280, A260/A230, Labeled Nucleic Acids, Microarray, dsDNA (Pro), ssDNA (Pro), RNA (Pro), Oligo DNA/Oligo RNA (Pro), RNA/DNA Deconvolution, Custom Factor (Pro); Protein A280 (Pro) and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins & Labels, dsDNA Fluorescence, RNA Fluorescence, Fluorometer. Protein Editor; Dye Editor, Kit Editor, OD600, Kinetics, UV-Vis, and Custom Methods |
| Language Support | Chinese, French, German, Japanese, Korean, Polish, Spanish, English, Italian | |
| Accessory Support | DYMO® LabelWriter® 550 Turbo printer, Bluetooth keyboard, mouse, and barcode reader | |
| Software Compatibility | Standalone, local control software | |
| NanoDrop Ultra PC control (free download) | ||
| Thermo Scientific SciVault 2 software (purchase separately) | ||
| PC Software: Windows® 10/11 Professional (64 bit) Acclaro Pro software (purchase seperately) |
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| Connectivity | 2x USB-A Ports, 1x USB-C Port, Ethernet, Bluetooth, Wi-Fi | |
| Advanced Connectivity | Google Drive | |
| Microsoft OneDrive | ||
| Thermo Fisher Connect Platform |
- SD of 10 individual samples measured at 0.97 A
- Absorbance expressed at Abs/mm at 25°C
- See NanoDrop Ultra dsDNA Broad Range (BR), NanoDrop Ultra dsDNA High Senstivitiy (HS), and NanoDrop Ultra RNA High Sensitivity (HS) Fluoresence Assays for more information.
- The MIQE Guidelines and Assessment of Nucleic Acids Prior to qPCR and RT-qPCR
- Measuring Viability Decline in Animal Cell Suspension Cultures Using the Thermo Scientific NanoDrop 2000 Spectrophotometer.
- Nanoparticle Characterization Resources for the NanoDrop 2000/c Spectrophotometer
- Quantification of Gold Nanoparticles
- Differences in Bacterial Optical Density Measurements between Spectrophotometers
- Activity Measurements of Pentose Phosphate Pathway Enzymes Using Kinetics
- Quality Control during Library Construction for de novo Next-Gen Sequencing Platforms
- Generation of a Standard Curve Based Custom Method using the NanoDrop 2000/2000c: Lowry Assay Case Study
- Quality Control of Industrial Dye Products Using Absorbance Spectroscopy
- Detection and Avoidance of Polysaccharides in Plant Nucleic Acid Extractions
- Quality Control of Small Molecule Pharmaceuticals using Spectrophotometry
- Pierce 660 nm Micro-Volume Assay Advantages
- Use of Custom Methods Capability of NanoDrop Spectrophotometers for Toxicology Studies
- Microvolume Quantification of Proteins by UV-Vis Absorbance or Fluorescence (Poster)
- Microvolume Quantification of Nucleic Acids in Molecular Diagnostics (Poster)
- Small Molecule Crystallography
- Microgenomic Expression Profiling
- Quantitative Real Time RT PCR
- Nucleic Acid and Fluorescent Dye Concentration Measurement
- HLA Typing: Template Quality Control for SSP and SSO
Acclaro Sample Intelligence Technology
Thermo Scientific Acclaro Sample Intelligence Technology software (included with NanoDrop Ultra spectrophotometers and fluorometers and NanoDrop Eight spectrophotometers) analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. It then applies powerful chemometric algorithms to find and correct for contamination in your samples. You get more reliable downstream assay results and avoid potential rework with this efficient, breakthrough capability.
Next-generation quality assessment using Acclaro software
Chemometric algorithms allow NanoDrop instruments to identify and correct for specific contaminants, taking quality assessment to the next level.
Acclaro Sample Intelligence software can even use full-spectrum data to tease apart DNA from copurified RNA and calculate corrected DNA concentrations for samples of mammalian, plant, and bacteria origin.
