Gemini XPS Microplate Reader
Develop new fluorescence assays without having to purchase expensive filter sets. The top-reading Gemini XPS Microplate Reader with dual monochromators lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for your new assays. Multiple-point well scanning optimizes cell-based assay sensitivity.
Benefits
- Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
- Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
- Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
- Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well
Measuring Accurately and Reproducibly
- Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
- Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
- Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
- Thorough mixing of samples in microplate wells by user-programmable Automix feature
- Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
- Seamless automation coupling with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for the SpectraMax Reader
Comprehensive Reader Validation
Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:
- SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
- SoftMax Pro Software validation package
- Software tools for FDA 21 CFR Part 11 compliance
SoftMax Pro Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software, offers:
- The most extensive library of supported application protocols to get you started faster
- Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
- Advanced calculations including non-linear fitting metrics for in-depth analysis
- The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
- Proven integrations with leading automation and LIMS platforms for partnered solutions
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General Specifications |
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| Dimensions (in.) | 8.6 H x 22.8 W x 15 D | |
| Dimensions (cm) | 22 H x 58 W x 38 D | |
| Weight | 35 lb (16 kg) | |
| Power source | 100-240 VAC, 50/60 Hz | |
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Performance Specifications |
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| Wavelength Range - Excitation | 250-850 nm | |
| Wavelength Range - Emission | 360-850 nm | |
| Wavelength Selection | Monochromator, tunable 1.0 nm increments | |
| Wavelength Bandwidth (Excitation and Emission) | 9 nm | |
| Wavelength Accuracy | < ± 2.0 nm | |
| Top Read Sensitivity | 3.0 fmol/well FITC 200 µl in 96 wells | |
| Light Source | Xenon flash lamp | |
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Microplate Read Time |
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| Normal Mode | 96 wells | 15 seconds |
| 384 wells | 45 seconds | |
| Dynamic Range | 6 orders of magnitude | |
| Temperature Range | Ambient + 4 °C up to 45 °C | |
| Temperature Uniformity (Microplate) | ± 1 °C @ 37 °C | |
The Gemini XPS Microplate Reader supports a multitude of fluorescence assay categories including:
- ELISAs and immunoassays
- Nucleic acid (DNA) quantitation
- Protein quantitation
- Reporter gene assays
- Cell viability, proliferation, and cytotoxicity
- Enzyme assays
- Transporter assays
- Phosphotases/kinases
- Microbial growth
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Product |
Product Number |
| Gemini XPS Microplate Reader | XPS |
| SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
| SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
| SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6267 |
| StakMax Microplate Handling Stacker | StakMax |
| Microplate Reader Shelf | 9000-0756 |
Brochure Gemini XPS Microplate Reader
Applications
- Live/Dead Assay® Viability/Cytotoxicity Assay for Cells Using the Gemini Fluorescence Microplate Reader
- A Stable, Sensitive Fluorescence-based Method for Measuring Cyclic AMP Using the Gemini Spectrofluorometer
- Measurement of Green Fluorescent Protein in the Gemini Spectrofluorometer
- Using NanoOrange Protein Kit with SpectraMax Microplate Readers
- Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Number of Citations*: 779
Latest Citations:
For a complete list, please click here.
Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor
BR Lee, DH Yi, E Song, SK Bhatia, JH Lee… - Applied biochemistry …, 2015 - Springer
... Two hundred microliters of the samples was mixed with 1 μL of the 1:1 mixture of SYTO®
9 and PI and incubated for 15 min in dark. The released fluorescence was measured using
a Gemini XPS fluorimeter (Molecular Devices, Sunnyvale, CA). ...
9 and PI and incubated for 15 min in dark. The released fluorescence was measured using
a Gemini XPS fluorimeter (Molecular Devices, Sunnyvale, CA). ...
[PDF] The chemokine platelet factor-4 variant (PF-4var)/CXCL4L1 inhibits diabetes-induced blood-retinal barrier breakdown
A El-Asrar, M Ahmed, G Mohammad… - … & visual science, 2015 - jov.arvojournals.org
... Fluorescence was measured using a spectraMax Gemini-XPS microplate reader
(Molecular Devices, Sunnyvale, CA, USA) with excitation and emission wavelengths
of 485 nm and 538 nm, respectively, with PBS as a blank. ...
(Molecular Devices, Sunnyvale, CA, USA) with excitation and emission wavelengths
of 485 nm and 538 nm, respectively, with PBS as a blank. ...
siRNA Delivery Impedes the Temporal Expression of Cytokine-Activated VCAM1 on Endothelial Cells
TT Ho, JO You, DT Auguste - Annals of Biomedical Engineering, 2015 - Springer
... of time were mixed with 100 µL of RNA detecting RiboGreen reagent (Life Technologies, Carlsbad,
CA, USA), incubated at RT for 5 min, then analyzed in 96-well plates using a fluorescence
microplate re
CA, USA), incubated at RT for 5 min, then analyzed in 96-well plates using a fluorescence
microplate re




























