Gemini EM Microplate Reader
Benefits
- Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes
- Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection between 250 and 850 nm
- Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading
- Up to 4 wavelength pairs can be read for endpoint and kinetic measurements
- Well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well
Measuring Accurately and Reproducibly
- Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics
- Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard
- Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
- Thorough mixing of samples in microplate wells by user-programmable Automix feature
- Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option
- Seamless automation coupling with the StakMax Stacker, Molecular Devices own integrated microplate stacker designed exclusively for the SpectraMax Reader
Comprehensive Reader Validation
Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:
- SpectraTest ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes
- SoftMax Pro Software Validation Package
- Software tools for FDA 21 CFR Part 11 compliance
SoftMaxPro Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software, offers:
- The most extensive library of supported application protocols to get you started faster
- Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
- Advanced calculations including non-linear fitting metrics for in-depth analysis
- The only Mac- and Windows-native application support for seamless exchange of data Files between operating systems
- Proven integrations with leading automation and LIMS platforms for partnered solutions
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General Specifications |
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| Dimensions (in) | 8.6 H x 22.8 W x 15 D | |
| Dimensions (cm) | 22 H x 58 W x 38 D | |
| Weight | 35 lbs (16 kg) | |
| Power Source | 100-240 VAC, 50/60 Hz | |
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Performance Specifications |
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| Wavelength Range-Excitation | 250-850 nm | |
| Wavelength Range-Emission | 250-850 nm | |
| Wavelength Selections | Monochromator, tunable 1.0 nm increments | |
| Wavelength Bandwidth (Excitation and emission) | 9nm | |
| Wavelength Accuracy | < ± 2.0 nm | |
| Top Read Sensitivity | 3.0 fmol/well FITC 200 µl in 96 wells | |
| Bottom Read Sensitivity | 8.0 fmol/well FITC 200 µl in 96 wells | |
| Light Source | Xenon flash lamp | |
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Microplate Read Time |
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Normal Mode |
96 wells | 15 seconds |
| 384 wells | 45 seconds | |
| Dynamic Range | 6 orders of magnitude | |
| Temperature Range | Ambient + 4°C up to 45°C | |
| Temperature Unitformity (Microplate) | ± 1°C @ 37°C | |
The Gemini EM Microplate Reader supports a multitude of fluorescence assay categories including:
- ELISAs and immunoassays
- Nucleic acid (DNA) quantitation
- Protein quantitation
- Reporter gene assays
- Cell Viability, proliferation, and cytotoxicity
- Enzyme assays
- Transporter assays
- Phosphotases/kinases
- Microbial growth
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Product |
Part Number |
| Gemini EM Microplate Reader | EM |
| SpectraTest FL1 Fluorescence Validation Plate | 0200-5060 |
| SpectraDrop™ Micro-Volume Microplate Starter Kit | 0200-6262 |
| SpectraDrop™ Micro-Volume Microplate HTS Kit | 0200-6267 |
| Microplate Reader Shelf | 9000-0756 |
Brochures Gemini EM Microplate Reader
Applications
- Measurement of Proteasome Inhibition in Live Cells inMolecular Devices Microplate Fluorometers
- Optimizing Excitiation and Emission Wavelengths for Narrow Stokes Shift Fluorophores Using the GEMINI and SoftMax Pro Software
- Selecting Excitation and Emission Wavelengths Using the GEMINI Microplate Spectrofluorometer - Basic Principles
- Measuring cell proliferation using the CyQUANT Cell Proliferation Assay with SpectraMax Microplate Readers
Number of Citations*: 1,340
[HTML] Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells
XL Lu, YH Lin, Q Wu, FJ Su, CH Ye, L Shi… - BMC complementary …, 2015 - biomedcentral.com
... Quantitative measurements of fluorescence readings were determined at 485/538
nm (excitation/emission) using a SpectraMax GEMINI EM fluorescent plate reader
(Molecular Devices, USA) from cells in 96-well plates. The ...
Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse Model
NN Naseri, H Xu, J Bonica, JPG Vonsattel… - … of Neuropathology & …, 2015 - journals.lww.com
Close Window. Close Window. Enter your Email address: Wolters Kluwer Health may email
you for journal alerts and information, but is committed to maintaining your privacy and will
not share your personal information without your express consent. ...
Oxidative stress involving changes in Nrf2 and ER stress in early stages of Alzheimer's disease
SI Mota, RO Costa, IL Ferreira, I Santana… - … et Biophysica Acta (BBA …, 2015 - Elsevier
Oxidative stress and endoplasmic reticulum (ER) stress have been associated with Alzheimer's
disease (AD) progression. In this study we analyzed whether oxidati.
The Mechanism of Melanocytes-Specific Cytotoxicity Induced by Phenol Compounds Having a Prooxidant Effect, relating to the Appearance of Leukoderma
T Nagata, S Ito, K Itoga, H Kanazawa… - BioMed research …, 2015 - hindawi.com
... The fluorescence inten




























