Echo 525 Liquid Handler For higher volume transfers
Liquid Handling Reimagined
Introducing the Echo 525 Liquid Handler, the first rapid acoustic liquid handler designed specifically for biochemical and genomics reagent transfer. The Echo 525 Liquid Handler and the Echo Software Applications enable scientists to spend more time looking at data. Protocol creation and implementation in the Echo Software Applications are fast and does not require extensive training. Designed for use with the Echo System, the Access Laboratory Workstation expands the Echo 525 Liquid Handler's utility and further frees up time.
Designed for biochemical and genomic sample and reagent transfer
Improve data quality while saving on reagent costs
Reagent reservoir for larger volume reagent transfers
Introducing the Echo 525 Liquid Handler, the first rapid acoustic liquid handler designed specifically for biochemical and genomics reagent transfer. The Echo 525 Liquid Handler and the Echo Software Applications enable scientists to spend more time looking at data. Protocol creation and implementation in the Echo Software Applications are fast and does not require extensive training. Designed for use with the Echo System, the Access Laboratory Workstation expands the Echo 525 Liquid Handler's utility and further frees up time.
Designed for biochemical and genomic sample and reagent transfer
Improve data quality while saving on reagent costs
Reagent reservoir for larger volume reagent transfers
Echo 525 Liquid Handler Features
Key Benefits
- Highly precise and accurate low volume transfers enabling assay miniaturization
- Builds assays 50–100 times faster than traditional liquid handlers giving the lab higher capacity from one instrument
- Suite of Echo Software Applications guide creation of complex protocols for a variety of applications
- Eliminates pipetting variances that affect assay success
- Integrates into the Labcyte Access Laboratory Workstation for increased throughput
Build Complex Assays Quickly
The flexibility, speed, accuracy, and precision of the Echo 525 Liquid Handler enable researchers to build assays that are not possible with traditional liquid handlers. The use of acoustic sound energy removes the traditional boundaries of tip and pin tool based liquid handlers. Transfer variable fluid heights and viscosities from one source plate to enable rapid assay creation in one fluid transfer run. Say goodbye to fixed plate designs as the Echo Liquid Handler moves fluids from any well to any well enabling researchers to design the experimental plates they need to make new discoveries.
Efficiently Transfer Build Reagents with Echo Qualified Reservoir
Assay assembly and the transfer of one reagent to many wells is simplified using the Echo Qualified Reservoir. With the capability of transferring up to 2.5 mL from each micro-well, you can build your assay without requiring an instrument change – saving time and money.
Flexible and Reliable Transfer of Genomic Reagents
The Echo 525 Liquid Handler transfers a wide range of fluids during one transfer including distilled water, buffers, nucleic acids, and reagents containing up to 50% glycerol. With its versatile fluid range, transfer volume range, any well to any well transfer capability, and powerful applications software the Echo 525 Liquid Handler supports a wide range of genomic assay workflows:
- Gene Synthesis
- PCR and qPCR assay set-up
- RNA and DNA NGS sequencing workflows
- Sanger sequencing assay set-up
- Single-cell sample preparation
Echo 525 Acoustic Liquid Handler Specifications
| Drop volume | 25 nL |
| Volume transfer range | 25 nL – 5 µL one well to one well |
| Transfer accuracy | <10% deviation from target volume |
| Transfer precision | <8% CV |
| Reagents supported | PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, and antibodies |
| Source labware compatibility | EchoQualified 384 Polypropylene, 384 Polypropylene Plus, Reservoir, Microplates |
| Destination labware compatibility | All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-well formats 8 -16 mm in height. Use of 1536-well plates as destination plates is application dependent |
| Dimensions | 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches) |
| Weight | 128 kg (283 lbs) |
| Working envelope | Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back |
| Electrical | AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A |
| Operating temperature | 21°C ± 5°C (70°F ± 9°F) |
| Vacuum | House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr (70.68 cu ft/hr), minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac) |
| Fluid class calibration | No calibration needed |
Echo Software Applications
Intuitive protocol design for Echo Liquid Handlers
Echo software solutions are tailored for a range of laboratory applications to enable users to maximize the utility of their Echo Liquid Handlers. Each application uses a combination of guided workflows and interactive graphics to simplify the creation and editing of complex protocols.
Echo 21CFR11 Compliance Manager
Echo Compliance Manager allows for the management and tracking of users, protocol changes, and electronic records associated with you Echo Liquid Handler
Echo Array Maker
Echo Array Maker simplifies custom array design to easily transfer samples and reagents from microplates to custom patterns on arrays, chips, and more
Echo Cherry Pick Software
Echo Cherry Pick software enables transfers of any volume of liquid from any source well into any destination well
Echo Combination Screen Software
Echo Combination Screening software for Echo Liquid Handlers simplifies the creation of combinatorial layouts to save time without limiting flexibility
Echo Dose-Response Software
Echo Dose-Response software provides a simple step-wise process for designing assay plate layout and creating dose-response curves
Echo Plate Audit Software
Echo Plate Audit software measures sample properties by performing acoustic analysis in order to validate and monitor sample libraries
Echo Plate Reformat Software
Echo Plate Reformat software offers a variety of liquid transfer functions, including assay plate replication, library compression, and customizable reformatting
TEMPO™ Automation Control Software
TEMPO™ Automation Control Software offers a dynamic interface for scheduling and managing tasks. Sample management, plate handling, liquid handling, and more
Access Laboratory Workstation
Combine the revolutionary performance of the Echo Liquid Handler with automated plate handling and integrated devices into a walk-away system tailored for a wide range of applications. Access Laboratory Workstations are modular, flexible solutions that easily scale when needed.
Access Laboratory Workstation Features
Automated Genomics Platform
Combine the revolutionary performance of the Echo Liquid Handler with automated plate handling and integrated devices to create a walk-away system tailored for a broad range of genomic applications. With use of the Access Workstation, Echo Liquid Handlers can be easily incorporated into an integrated system to automate genomic workflows including, qPCR, PCR, enzyme reactions, DNA/RNA quantification, sample pooling, and normalization.Assay-Ready Plate Preparation
The Access Laboratory Workstation for Assay-ready Plates incorporates a sealer, peeler, bulk-dispenser, and centrifuge to support walk-away library reformatting, replication, cherry-picking and dose-response. This workstation accommodates all Echo Qualified Plates and most ANSI-compliant/SBS-standard microplates. With random access microplate storage and options for barcode reading, shaking and de-lidding, the Access Laboratory Workstation offers the productivity of a large-scale system in a compact workspace.Lead-Optimization and Secondary-screening Assays
Option to integrate multi-mode microplate readers and controlled storage systems that address a wide range of biochemical and cell-based assays into a standard Access Laboratory Workstation configuration. This flexibility enables walk-away automation for Cytochrome P450 screening, cell signaling, cell viability, and other types of assays. Storage systems can be configured for cold-dry compound storage, warm humid cell storage, or ambient storage.High-Throughput Gene Expression Assays
The Access Laboratory Workstation, combined with a one-step qPCR preparation workflow for RT-qPCR can be adapted for high-throughput gene expression screening. With its ability to transfer nanoliter (nL) volumes of samples and reagents accurately and precisely, the Echo Liquid Handler dramatically reduces screening costs — making one-step RT-qPCR viable as a high-throughput screening method. To maximize efficiency, the Access Laboratory Workstation incorporates two Roche LightCycler systems with the Echo Liquid Handler and other devices for cell-dispensing, washing, centrifugation, and sealing.Automated Protein Crystallography Setup
The Access laboratory workstation for protein crystallography applications incorporates an adhesive sealer to prevent evaporation and eliminate the risk of heat exposure, which can occur with thermal sealing. Random access to reagent plates enables on-the-fly creation of grid screens from high-concentration stocks.Analysis Only
A single Access Laboratory Workstation is capable of integrating several detection devices and pooling them to create an automated analysis system that can handle racks of assay plates. Additional accessories, including waste chutes, barcode readers, and lid removal stations can also be integrated into the workstation.Access Dual Robot System
The Access Dual Robot System (DRS) is a compact, modular, high-capacity, and high-throughput platform, designed and optimized for the use of Echo Liquid Handlers in sample management workflows. It is the only automation platform on the market compatible with Echo® 650 Series Liquid Handlers, enabling the production of assay-ready plates from acoustic sample tubes. The system combines industry-leading devices and software with ergonomic and environment management options to provide a complete, easy-to-use, store-to-assay sample management solution.
