Digidata 1550B plus HumSilencer Feature, Patch Clamp, Elektrofiziológia
For a variety of electrophysiology recordings including single-channel recordings, whole-cell voltage- and current-clamp recordings, intracellular sharp electrode recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies.
What Is the HumSilencer Feature?
The HumSilencer feature is advanced technology that learns the local line-frequency noise patterns and associated high-frequency harmonics and removes them from the incoming signal in a filter-free way. Controlled through a simple ON/OFF check box in the Axon AxoScope or Axon Clampex software, the HumSilencer is easy to use, enabling (or disabling) subtraction of line-frequency noise from your signal with a single click.
If the pattern of the line frequency noise changes, such as by the introduction of a new noise source or through an increase in existing noise, HumSilencer rapidly learns and adapts. The new noise is eliminated in less than one second, and for amplitudes at the digitizer’s analog input of up to 20 V, peak-to-peak.
The HumSilencer feature is not a filter and does not have a filtering effect on acquired signals; nor does the HumSilencer system cause signal distortion, such as frequency change, amplitude attenuation, phase shift, or DC voltage change. It is an adaptive, built-in solution to the problem of line-frequency noise.
With the Digidata 1550A plus HumSilencer, you get all the functions and capabilities of the Digidata 1550, with the added ease and convenience of the powerful HumSilencer
Features
- Eliminates 50/60 Hz line-frequency noise and associated high frequency harmonics in less than one second and with a single click
- Digitizes a wide range of input signals from -10 to +10 V
- Eliminates a maximum noise amplitude of up to 20 V, peak-to-peak
- 8 analog input channels to digitize acquired signals up to 500 kHz independently
- 8 analog output channels to send command voltage output independently
- 8 digital out channels to control the periphery equipment used in sophisticated experiments
- Independent analog-to-digital converters for each input channel ensure low crosstalk levels and high data acquisition rates.
- USB 2.0 connection allows operation on virtually any current PC computer running Microsoft Windows 7 (32-bit or 64-bit) Operating System, including laptops.
- 16-bit resolution data
- State-of-the-art signal-to-noise ratio
- All signal connectors on the front panel for ease of access and maintenance of the electrophysiology set-up
- Rack-mount case for tidy installation of all components in the electrophysiology set-up
- User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference.
General Specifications
| General Specifications | |
|
HumSilencer |
|
| Maximum input signal (total of noise + signal) |
±10 V |
| Maximum noise amplitude | 20 V, peak-to-peak (on a 0 V signal) |
| Noise cancellation | Line-frequency (50 Hz / 60 Hz) and harmonics to 10 kHz |
| Cancellation response time | < 1 second |
|
Analog Inputs |
|
| Number of input channels | 8 single-ended |
| Number of ADCs | 8 |
| Sampling rates |
1 Hz - 500 kHz; Maximum aggregate throughput rate is 500 kHz x 8 input channels = 4 Megasamples/s
|
| Resolution | 16-bit |
| Input range | -10.000 V to +10.000 V |
| Input resistance | >1 MΩ |
| Gain value | 1 |
|
Analog Outputs |
|
| Number of channels | 8 |
| Number of DACs | 8 |
| Sampling rates | 1 Hz - 500 kHz |
| Resolution | 16-bit |
| Output range | -10.000 V to +10.000 V |
| Output impedance | < 0.5 Ω |
| Output short circuit to signal ground | ±25 mA |
|
Digital Outputs |
|
| Number of bits | 8 (of 16) supported in software |
| SCOPE | Dedicated trigger output |
| Output driver | Advanced CMOS (AC) compatible |
| Output current | ±4 mA source, cable |
| Type | USB 2.0 braided |
| Length | 3 meters |
|
Trigger Inputs |
|
| Input type | TTL compatible |
| TAG | Rising-edge sensitive |
| START | Rising-edge sensitive |
| Maximum Current* | ||||||
| I-Clamp | HVIC | TEVC | dSEVC | DCC | ||
| Headstage | Ro | (Ch1 &2) | (Ch2) | (Ch2) | (Ch1) | (Ch1) |
| HS-9Ax0.1U | 100M | 0.12μA | 1.8μA | 1.8μA | 0.036μA | 0.036μA |
|
HS-9Ax1U
|
10M | 1.20μA | 18.0μA | 18.0μA | 0.360μA | 0.360μA |
| HS-9Ax10U | 1M | 12.00μA | 180.0μA | 180.0μA | 3.600μA | 3.600μA |
*Maximum current specifications assume negligible electrode resistance.
| Description | Details | Part Number |
| Axoclamp 900A Headstage HS-9A X0.1U | x0.1 headstage | 1-2950-0359 |
| Axoclamp 900A Headstage HS-9A X1U | x1 headstage | 1-2950-0360 |
| Axoclamp 900A Headstage HS-9A X10U | x10 headstage | 1-2950-0361 |
| Axoclamp 900A Headstage VG-9A X10U | x10 virtual ground headstage | 1-2950-0362 |
| Axoclamp 900A Headstage VG-9A X100U | x100 virtual ground headstage | 1-2950-0363 |
SoftPanel Interface
To offer a more conventional interface to amplifier control, the optional SoftPanel was designed as a hardware extension to replicate all essential amplifier functions by acting as a hardware extension to the Axoclamp and MultiClamp Commander Software. The SoftPanel controller communicates with the computer via an easy-to-set up USB 2.0 connection. Using the SoftPanel knobs replicate continuous mouse controls ("gliders"), while buttons replicate single-click mouse controls without negating the many benefits afforded by computer control of the amplifier.
Axoclamp Commander Software
Instead of the traditional front panel knobs and switches, the Axoclamp 900A Amplifier is controlled by the Axoclamp 900A Commander Software, a program that runs on a Windows PC computer (see system requirements) and communicates with the amplifier via a USB interface. This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization for improved ease of use. In addition, Axoclamp Commander has "smart" features that protect cells from potentially damaging signal oscillations and automate mode changes based on internal signal thresholds or externally applied signals. Amplifier settings such as gain, filter frequency, recording mode, and input/output scale factors are automatically telegraphed to the pCLAMP 10 Data Acquisition Software via the USB connection. This ensures that the amplifier settings are accurately and conveniently stored in the recorded data files.
To obtain the installer for a more in-depth look at Axoclamp 900A Commander Software, please go to our Customer Support page.
In combination with a Digidata 1550 Data Acquisition System and pCLAMP Software, the Axoclamp 900A Amplifier forms the core of a recording setup like those used in many electrophysiology labs around the world. Computer control of the amplifier ensures correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module in pCLAMP Software makes optimal use of the features of the Axoclamp 900A Amplifier.
